Bacterial Transformation Lab ReportTitle:Objective(s):Hypothesis:Materials:Summary of Steps?Bacterial transformation lab? Can the length of heat shock during incubation affect plasmid transfer?? Bacterial Transformation Virtual Lab?1. Fill all three tubes?. Place tubes into ice bath?. Inoculate each tube?. Put a colony in each tube?. Swirl the rube?. Record data?ariables:?ontrolled variables: tube and solution?ndependent variable: ice bath?ependent variable: colony?ata:?able 1: Can the length of heat shock during incubation affect plasmid transfer?Image transcription textSample 1 Sample 2 Sample 3 length of heat shock 50 sec 90 sec 0 (seconds) number of colonies 87 57 28Sample 1 Sample 2 Sample 3 Volume of starting plasmids (in uL) number of colonies… Show moreVolume of calcium chloride: 250?Volume of plasmid solution: 50, 90, 0?Concentration of plasmid solution: 10?Volume of LB nutrient broth solution: 250?Volume of transformation sample transferred to the agar plate: 100?alculate the transformation efficiency of each of your three bacteria samples. For guidance on these calculations, review the steps and example provided in the pre-lab activity of the lesson.?ample 1:?ample 2:?ample 3:?onclusion:?rite a conclusion statement that addresses the following questions:?How did different plasmid concentrations of heat shock periods affect the transformation efficiency of your bacteria samples??hat evidence suggests that the changes were due to the transformation procedures you performed??oes your data support or fail to support your hypothesis (include examples)??ab Reflection Questions?. What barriers might hinder the acquisition of plasmids??. How did the addition of CaCl2 and “heat shocking” facilitate the introduction of plasmids into the E. coli cells??. What advantage would there be for an organism to be able to turn on or off gene expression in response to certain conditions?BiologyScienceGeneticsAP BIOLOGY 8724

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